You only need one Optionbutton really. If your Optionbutton1 controls negative amounts the something like this (change the code to write to your sheet).

Private Sub CommandButton1_Click()
    If Me.OptionButton1 Then
        MsgBox Val(Me.TextBox1.Value) * -1
    Else: MsgBox Val(Me.TextBox1.Value)
    End If
End Sub
Note iy you declare Tot as long then you will not be able to use decimals.